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Cell membranes room semipermeable barriers, and osmotic gradients room established between intracellular and extracellular fluids i m sorry can reason water to circulation into and out of the cells. The amount of osmotic pressure depends ~ above the difference between the concentration of non-diffusible ions on every side the the membrane.
The theoretical background for this practice is to be reviewed in your text book.

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The intracellular liquid of erythrocytes is a systems of salts, glucose, protein and also hemoglobin. A 0.9% NaCl solution is claimed to be isotonic: as soon as blood cells reside in together a medium, the intracellular and also extracellular fluids space in osmotic equilibrium throughout the cell membrane, and also there is no network influx or efflux the water.
as soon as subjected to hypertonic media (e.g. 1.8% NaCl), the cells shed their normal biconcave shape, experience collapse (leading to crenation) as result of the rapid osmotic efflux the water. On the various other hand, in a hypotonic atmosphere (e.g. 0.4% NaCl or distilled water), an influx of water occurs: the cell swell, the integrity of their membranes is disrupted, allowing the escape of your hemoglobin (hemolysis) which dissolves in the outside medium.

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Crenation
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Hemolysis
In this experiment, we make use of the residential property that the osmotic fragility (or susceptibility come hemolysis) of erythrocytes is not uniform, and also the number of cells undergoing hemolysis counts on the level of hypotonicity the the extracellular medium. The concentration that liberated hemoglobin in each test tool is an table of contents of the extent of osmotic hemolysis. Your task is to examine the relationship in between extent of hemolysis and also osmolarity the the tool in which the erythrocytes room suspended.
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